Microsatellite Sequences for Pacific Threadfin Identification and Parental Assignment

Description

Applications

• The technology can be used for Pacific Threadfin parent assignment.  In a hatchery operation with many male and females, by genotyping all the brookstock, offsprings from the broodstock can be assigned to their parents.  By using single larvae and its DNA, the technology can identify the original female of the larvae.  For effective broodstock management, frequent spawning females can be identified.  The spawning patterns of the female broodstock in a given population can be established by analyzing the microsatellite loci with samples from several consecutive spawn events.

• The technology can be used for identifying or tracing origins of Pacific Threadfin products.  Genomic DNA can be isolated from samples to be tested.  A panel of DNA markers can be selected and polymerase chain reaction is applied for specific amplifications of the DNA markers.  By agarose gel electrophoresis of DNA fragment analysis, presence of the DNA fragments can be identified to determine whether the product origins from Pacific Threadfin, and the possible sites of production.

• The identified Pacific Threadfin DNA markers is also useful as a linkage mapping tool in identifying the genes or quantitative trait loci that are important for Pacific Threadfin aquaculture such as growth performance, diease resistances and reproduction.

• The identified Pacific Threadfin DNA markers can be used as a genetic testing tool to distinguish genetic differences or composition among different populations of Pacific Threadfin in the wild and captive broodstock.

Main Advantages

Inventor(s)

Jinzen Yang
Heng Wang
Zhao Baopinig

Human Nutrition, Food and Animal Science, College of Tropical Agriculture and Human Resources

Contact Information

For licensing information, please contact Lisa Matsunaga at matsunag@hawaii.edu